Journal: Microbiology Resource Announcements
Article Title: Genome Sequence of Citrobacter freundii AMC0703, Isolated from the Intestinal Lumen of an 11-Year-Old Organ Donor
doi: 10.1128/MRA.00994-20
Figure Lengend Snippet: Scanning electron microscope (SEM) imaging of Citrobacter freundii AMC0703. Briefly, bacterial cell pellets were resuspended in 2% paraformaldehyde/2.5% glutaraldehyde in 0.15 M sodium phosphate buffer, pH 7.4. Following treatment as described previously , the fixed cell suspension was deposited onto 12-mm round poly- d -lysine-coated coverslips, and following preparation, these were mounted on 13-mm aluminum stubs and sputter coated with 5 nm of a gold-palladium alloy. A Zeiss Supra 25 field emission SEM operating at 5 kV was used to view the AMC0703 isolate with scanning electron microscopy.
Article Snippet: AMC0703 shares 98.77% ANI to the type strain C. freundii ATCC 8090.
Techniques: Microscopy, Imaging, Suspension, Electron Microscopy